6 SAMPLE ANALYSIS TOOLS for VOCs, SVOCS, and PESTICIDES
6.2 EX-SITU ANALYSIS
6.2.20 Immunoassay Colorimetric Kits
||Field screening of individual contaminants.
See 7.1.6 for
use with metals and 9.1.8 for use with explosives.
Immunoassay technology relies on an antibody that is developed to have a high degree of
sensitivity to the target compound. This antibody's high specificity is coupled within a
sensitive colorimetric reaction that provides a visual result. The intensity of the color
formed is inversely proportional to the concentration of the target analyte in the sample.
The absence/determination is made by comparing the color developed by a sample of unknown
concentration to the color formed with the standard containing the analyte at a known
|1. Non-Halogenated VOCs
||2. Non-Halogenated SVOCs
||3. Halogenated VOCs
||4. Halogenated SVOCs
||Technique measures the contaminant indirectly.
|Susceptibility to Interference:
|Detection Limits :
||10-100 ppm (soil); 0.5-10 ppm (water).
|Turnaround Time per Sample:
|Quantitative Data Capability:
||Data become quantitative with additional effort.
||Commercially available technology with moderate field experience.
||Technology has participated in CalEPA certification and/or CSCT
|Relative Cost per Analysis:
Kits: $10-$25 per sample - does not include equipment.
- Not applicable for all classes of small, environmentally significant molecules; for
example, long chain aliphatic hydrocarbon compounds and most transition metals.
- Temperature extremes can affect test results; the operational temperature ranges are
typically between 40°F and 100°F.
- Can be affected by specific matrix conditions. For example, analyte recovery for
analytes like PCB and PAH can be affected by excessive soil moisture.
- Percent levels of oil can result in false positive results.
- Prior knowledge of analytes and potential interferences strongly recommended.
||A11 Methods for Specific Analytes.